Diarrhea Research Today is a free monthly online journal that collates and summarizes the latest research about Diarrhea, including details on infant and child diarrhea, causes, treatment, pregnany.

An ELISA optimized for porcine epidemic diarrhoea virus detection in faeces.

Rodák L, Valícek L, Smíd B, Nevoránková Z

Veterinary Research Institute, Department of Virology, Hudcova 70, 621 32 Brno, Czech Republic. rodak@vri.cz

Monoclonal antibodies to porcine epidemic diarrhoea virus (PEDV) membrane protein M were prepared and used for the comparative assessment of three blocking ELISA variants to detect PEDV. The competitive blocking ELISA (CB-ELISA) format showed the highest sensitivity, allowing detection of 10(2.5) plaque-forming units of PEDV/ml in culture medium. Its specificity was verified by inclusion of control samples containing transmissible gastroenteritis virus (TGEV) and rotavirus A in each analysis. Eighty porcine field samples of faeces obtained from 38 herds affected with diarrhoea were examined, and PEDV was found in 15 (19%) samples from 6 (16%) herds. The suitability of the CB-ELISA for the screening herds in epizootiologic situations is discussed.

Published 20 December 2004 in Vet Microbiol, 105(1): 9-17.
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